Description
The human IL13RA1 / CD213a1 ELISA Pair Set is for the quantitative determinationof human IL13RA1. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL13RA1 /CD213a1 coated on a 96-well plate. Standards and samples are added to thewells, and any IL13RA1 / CD213a1 present binds to the immobilized antibody. Thewells are washed and a horseradish peroxidase conjugated mouse anti-IL13RA1monoclonal antibody is then added, producing an antibody-antigen-antibody"sandwich". The wells are again washed and TMB substrate solution is loaded,which produces color in proportion to the amount of IL13RA1 present in thesample. To end the enzyme reaction, the stop solution is added and absorbancesof the microwell are read at 450 nm